Native Colorado River cutthroat trout were restocked along the upper section of the East Fork of Hermosa Creek in June 2023. Twenty-three miles of the upper Hermosa Creek and the East Fork are isolated from the rest of the watershed and have previously been restored as Colorado River cutthroat trout waterways beginning in the late 1980s. Prior to the 2023 restocking, environmental deoxyribose nucleic acid (eDNA) samples were collected from the upper mainstem and East Fork of Hermosa Creek. The population of Colorado River Cutthroat Trout is greater than ninety percent pure and a targeted conservation population. Colorado Parks and Wildlife (CPW), Trout Unlimited volunteers, and Forest Service employees released Colorado River cutthroat trout, restocking the upper section of the East Fork of Hermosa Creek resulting in nearly 8 miles of stream habitat enhanced.